HPLC Analysis of Sulfonamide on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Sulfonamide on Chromolith® High Resolution RP-18e


column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 m (1.52022.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] water + 0.1%TF
gradient 98% B held for 1 min; to 85% B for 3.2 min; held at 85% B for 6.8 min; (Initial composition: 0-1min 98%B, 1-3,2min 98%B to 85%B, hold 85%B until 10min )
flow rate 4.0 mL/min
sample Sulfadiazine 1,1mg/ml, Sulfamethoxazole 0,8mg/ml, Sulfadimethoxine 0,8mg/ml, Sulfaquinoxaline 1,0mg/ml, Sulfadimidin 2,6mg/ml, Sulfamerazine 1,0mg/ml, Sulfathiazole 1,5mg/ml, Sulfonilamid 0,7mg/ml, Sulfisoxazole 1,0mg/ml
injection 5 μL
detector 270 nm cell: 11μL


Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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