HPLC Analysis of Sulfonamides in Honey on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Sulfonamides in Honey on Chromolith® High Resolution RP-18e

Conditions

sample preparation 2 g honey dissolved in 10 mL 100% mobile phase A acetonitrile:20 mM ammonium acetate, pH 4.75,  2:98 v/v), homogenized in an ultrasonic bath, filtered through 0.2 μm filter prior to analysis
column Chromolith® High Resolution RP-18 encapped, 100 x 4.6 mm (1.52022.0001)
column temp. 40 °C
mobile phase [A] acetonitrle / 20mM Ammoniumacetate pH=4.75  2/98 v/v; [B] acetonitrle / 20mM Ammoniumacetate pH=4.75  32/68 v/v;
gradient 0-20.7min 22%B to 35%B ; 20.7-27.3min 35%B to 75%B ; 27.4-32min 95%B
flow rate 0.7 mL/min
pressure 522 psi (36 bar)
sample Honey 2g/10mL (Diluent: Honey: 100% A)
injection 5 μL
detector 270 nm (cell: 11 μL)

Description

Analysis Note Sample: 2 g honey dissolved in 10 mL mobile phase A (acetonitrile:20 mM ammonium acetate, pH 4.75,  2:98 v/v), homogenized in an ultrasonic bath, filtered through 0.2 μm filter prior to analysis
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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