HPLC Analysis of Tolvaptan Assay on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Tolvaptan Assay on Chromolith® High Resolution RP-18e


column Chromolith® High Resolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)
column temp. 40 °C
mobile phase [A] 1.15 g of ammonium dihydrogen phosphate & 1.32 gm of di-ammonium hydrogen phosphate 1L Milli-Q® water. (pH is about 6.8); [B] acetonitrile; (75:25, A:B)
flow rate 2.0 mL/min
sample Weigh 25 mg substance in 50 ml volumetric flask. Dissove in diluent & make up the volume with diluent - 1 ml of this solution diluted to 10 ml with diluent..
injection 10 μL
detector 265 nm cell: 10 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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