HPLC Analysis of Tryptic Peptides from Ovalbumin on SeQuant ZIC-HILIC

HPLC Analysis of Tryptic Peptides from Ovalbumin on SeQuant ZIC-HILIC

Conditions

column SeQuant ZIC-HILIC, PEEK, 150 x 0.3 mm, 5 μm, 200 Ã… (1.50481.0001)
mobile phase [A] 0.25% (v/v) formic acid with Milli-q-water; [B] 0.25% (v/v) formic acid in acetonitrile; (10:90, A:B)
gradient Initial composition: 90% B and 10% A. Linear gradient from 0-40 min
flow rate 5 μL/min
injection 1 μL of 10 pmole digested sample injected in mobile phase
detector MicroMass Ultima-QTOF, Needle voltage: 3.1 kV, Cone voltage: 45V (collision energy: 10V; Scan range: m/z 400-1800; 1.5 s cycle time)

Description

Featured Industry Life Science and Biopharma
suitability application for HPLC

Materials

     
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