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HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL(TM) LC-18

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18


column SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase [A] methanol: [B] deionized water (98:2, A:B)
flow rate 2 mL/min
sample 0.2-1 mg/mL each analyte in methanol
injection 20 μL
detector UV, 325 nm (retinol, retinyl acetate) or 290 nm (tocopherols, tocopherol acetate)


Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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