HPLC Analysis of Vitamins, Fat Soluble, on Ascentis® Si

HPLC Analysis of Vitamins, Fat Soluble, on Ascentis® Si

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 30 °C
mobile phase [A] hexane; [B] ethyl acetate
gradient 10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min
flow rate 1.0 mL/min
sample as indicated in 96:4 (hexane:isopropanol)
injection 10 µL
detector UV, 290 nm

Description

Analysis Note Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).
Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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