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HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® RP-Amide

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 °C
mobile phase [A] 20 mM phosphoric acid (pH 2.0 unadjusted): [B] methanol; (30:70, A:B)
flow rate 1.0 mL/min
sample as indicated in 20 mM phosphoric acid (pH 2.0)
injection 10 µL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Anticoagulants
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Warfarin is a trademark of Wisconsin Alumni Research Foundation
suitability application for HPLC

Materials

     
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