HPLC Analysis of Xanthines on Purospher®STAR RP-18 endcapped


column PurospherSTAR RP-18 endcapped, 10 cm x 3.0 mm, 3 µm particles
column temp. 35 °C
mobile phase [A] 0.1% formic acid in water [B] 0.1% formic acid in methanol
gradient 2.5% [B] held for 2 min; to 40% [B] in 10 min
flow rate 0.6 mL/min
pressure 2500 psi (170 bar)
sample 50 µg/mL in 60:40, water:methanol
injection 1 µL
detector UV, 270 nm


Analysis Note Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators, notably in the treatment of asthma symptoms. This application shows the efficient separation of several common xanthines and may be applied their analysis in any number of desired matrices.
Categories Analytical Chromatography, Stimulants, Antiasthmatics, Brochodilators
Featured Industry Clinical
Food and Beverages
Forensics and Toxicology
Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


Related Links