HPLC Analysis of Xiaoyao Wan on Chromolith(R) HighResolution RP-18 endcapped

HPLC Analysis of Xiaoyao Wan on Chromolith® HighResolution RP-18 endcapped


column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 0.1% H3PO4 aqueous solution; [B] acetonitrile; (85:15, A:B)
flow rate 1.0 mL/min
pressure 826 psi (57 bar)
sample Dilute ethanol solution (dilute 529 mL ethanol with water to 1000 mL). (60 μg/mL (60 ppm) Paeoniflorin in diluent.)
injection 10 μL
detector 230 nm cell: 1μL/10mm


Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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