HPLC Analysis of Xiaoyao Wan Tablets on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Xiaoyao Wan Tablets on Purospher® STAR RP-18 endcapped


sample preparation Take appropriate amount of drug pills, grind, weigh 0.4g powder a volumetric flask, add into 25 mL dilute ethanol aqueous solution, weigh the solution, sonicate (250w, 33kHZ) for 30minutes, cool, weigh, make up weight with diluent. Shake and filter.
column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
mobile phase [A] 0.1% H3PO4 aqueous solution; [B] acetonitrile; (85:15, A:B)
flow rate 1 mL/min
pressure 1537 psi (106 bar)
sample Dilute ethanol solution (dilute 529 mL ethanol with water to 1000 mL). (60 μg/mL (60 ppm) Paeoniflorin in diluent.)
injection 10 μL
detector 230 nm cell: 1μL/10mm


Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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