HPLC Analysis of Xiaoyao Wan Tablets on Chromolith(R) HighResolution RP-18 endcapped

HPLC Analysis of Xiaoyao Wan Tablets on Chromolith® HighResolution RP-18 endcapped

Conditions

sample preparation Take appropriate amount of drug pills, grind, weigh 0.4g powder a volumetric flask, add into 25 mL dilute ethanol aqueous solution, weigh the solution, sonicate (250w, 33kHZ) for 30minutes, cool, weigh, make up weight with diluent. Shake and filter.
column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 0.1% H3PO4 aqueous solution; [B] acetonitrile; (85:15, A:B)
flow rate 1 mL/min
pressure 826 psi (57 bar)
sample Dilute ethanol solution (dilute 529 mL ethanol with water to 1000 mL). 60 μg/mL (60 ppm) Paeoniflorin in diluent.
injection 10 μL
detector 230 nm cell: 1μL/10mm

Description

Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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