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LC/MS Analysis of Amino Acids on Ascentis® Express HILIC

LC/MS Analysis of Amino Acids on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U)
column temp. 35 °C
mobile phase [A] 13 mM ammonium acetate in 10:90 (v/v) water: acetonitrile; [B] 13 mM ammonium acetate in water
gradient held at 0% B for 1 min; 0 to 90% B in 19 min
flow rate 1.0 mL/min
sample 10 mg/L in Mobile Phase A
injection 10 µL
detector ESI(+)

Description

Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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