LC/MS Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC

LC/MS Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC


column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid
flow rate 0.4 mL/min
pressure 1305 psi (90 bar)
sample each compound, 300 ng/mL in 75:25 (1% formic acid acetonitrile:water)
injection 0.5 µL
detector ESI+, 100-1000 m/z


Analysis Note The basic nature of these antiarrhythmic agents makes them ideal candidates for HILIC (hydrophilic interaction) chromatographic separation. The benefit of HILIC over traditional reversed-phase chromatography is two-fold for both sample introduction and analyte detection. First, the high acetonitrile concentration of HILIC mobile phases allows for direct analysis of precipitated plasma samples without the need for additional sample solvent exchange. Second, the high acetonitrile content provides increased analyte response in positive ESI MS detection. Of the various HILIC-mode columns tested, method development for this assay determined the Ascentis Express HILIC, 2.7 μm particles, provided the best chromatographic resolution of the antiarrhythmic drugs while maintaining high peak efficiency for enhanced detection levels.
Categories Analytical Chromatography, SPE Applications, Clinical, antiarrhythmic drugs, cardiac drugs, Antiarrhythmic drugs, Cardiac Drugs, Cardiac drugs
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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