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LC/MS Analysis of Benzodiazepines on Ascentis® Phenyl (Analyte Mix 1)

LC/MS Analysis of Benzodiazepines on Ascentis® Phenyl (Analyte Mix 1)


column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 °C
mobile phase [A] 0.1% ammonium acetate in water (pH unadjusted), [B]: 0.1% ammonium acetate in acetonitrile, (50:50, A:B)
flow rate 1 mL/min., split to the MS
sample as indicated in 0.1% ammonium acetate in 90:10:water:acetonitrile
injection 5 µL
detector MS, ESI (+) in selected ion recording (SIR) mode


Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, psychotropics, Anticonvulsant/antiepiletic/antiseizure, anxiolytics/sedatives, antiepileptic/antiseizure, Anxiolytics, Sedatives
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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