LC/MS Analysis of Combination Drug Panel on Ascentis(R) Express Biphenyl

LC/MS Analysis of Combination Drug Panel on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 5 cm x 2.1 mm I.D., 2.7 μm particles (64057-U)
column temp. 25 °C
mobile phase [A] 5 mM ammonium formate, 0.1% formic acid in water; [B] 5 mM ammonium formate, 0.1% formic acid in water:methanol (5:95)
gradient 5% B for 1 min; to 100% B in 5 min; held at 100% B for 1 min
flow rate 0.5 mL/min
pressure 431 psi (29.8 bar)
sample 300 ng/mL in urine after dilution 9:1 in water
injection 1 μL
detector MS, ESI+, EIC

Description

Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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