LC/MS Analysis of D-Mannose on SeQuant ZIC-HILIC

LC/MS Analysis of D-Mannose on SeQuant ZIC-HILIC

Conditions

column SeQuant ZIC-cHILIC, PEEK, 150 x 4.6 mm, 5 μm, 100 Ã… (1.50661.0001)
column temp. 40 °C (oven), 40 °C (detector cell)
mobile phase [A] acetonitrile; [B] 3.54 g of ammonium acetate in 500 ml milliQ water; (80:20, A:B)
flow rate 0.5 mL/min
pressure 421 psi (29 bar)
sample see Description text
injection 10 μL
detector Shimadzu Prominence, R.I.

Description

Analysis Note Pipette out 2.5 ml of suspension into a 50 ml volumetric flask. Add 30 ml of diluent. Sonicate for 10 minutes. Centrifuge the solution at 10000 rpm for 10 minutes. Filter through 0.2 um filter paper.
Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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