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LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2

LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2


column apHera NH2, 15 cm x 4.6 mm I.D., 5 µm particles (56401AST)
column temp. 30 °C
mobile phase [A] water; [B] 20mM ammonium carbonate, pH 9.0
gradient 100% A for 0.5 min; to 100% B in 0.1 min; held at 100% B for 9.4 min; to 100% A in 0.1 min; held at 100% A for 5 min
flow rate 0.5 mL/min
pressure 997 psi (69 bar)
sample 50 ng/mL each compound in 50:50 methanol:water with 0.05% formic acid
injection 2 x 20 µL
detector ESI-MS(-); MS/MS 168/63; 110/63; 180/63


Analysis Note An LC/MS method for analysis of glyphosate, AMPA and glufosinate using MS-compatible mobile phase on an ion-exchange pH-stable HPLC column with mass spectrometry detection is shown here.
Categories Analytical Chromatography, Herbicides
Featured Industry Food and Beverages
Legal Information apHera is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS


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