LC/MS Analysis of Metformin and Related Impurities on SeQuant(R) ZIC-HILIC

LC/MS Analysis of Metformin and Related Impurities on SeQuant® ZIC-HILIC

Conditions

column SeQuant® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Ã… (1.50661.0001)
column temp. 30 °C
mobile phase [A] acetonitrile; [B] 4.62 g of ammonium acetate in 1000 ml water (60 mM). [A] acetonitrile; [B] 4.62 g of ammonium acetate in 1000 ml water (60 mM). Adjust buffer to pH 5 using glacial acetic acid; (90:10, A:B)
flow rate 1.5 mL/min
pressure 1522 psi (105 bar)
sample 5000 ppm metformin & 1 ppm of each impurity: A, C and melamine & 5 ppm of impurity B in mobile phase
injection 5 μL
detector 218 nm cell: 8 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

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