LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC – Effect of Mobile Phase Buffer Concentration

LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC – Effect of Mobile Phase Buffer Concentration

Conditions

instrument Agilent® 6530 Q-TOF LC/MS
column SeQuant ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 µm
mobile phase   [A] acetonitrile; [B] 50 mM or 100 mM ammonium acetate, pH 5.3 with acetic acid in water
gradient 26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 4.9 min
flow rate 0.3 mL/min
pressure 1125 psi
column temp. 40 °C
detector Q-TOF (ESI-negative)
detector other XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, CTP-482, GMP-362,  GDP-442,  GTP-522, UMP-323, UDP-403, UTP-483
injection 10 µL

Description

Analysis Note Adjusting buffer concentration and the ionic strength can alter retention and selectivity in HILIC. The buffer salts in HILIC mobile phases are important in reducing electrostatic interactions (attraction and repulsion) between charged analytes and the stationary phase.
Featured Industry Life Science and Biopharma 
Legal Information SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
LiChrosolv is a trademark of Merck KGaA, Darmstadt, Germany
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Application No. ASC140218H

Materials

     
Related Links