LC/MS Analysis of Nucleotides SeQuant ZIC-cHILIC – Effect of pH

LC/MS Analysis of Nucleotides SeQuant ZIC-cHILIC – Effect of pH

Conditions

instrument Agilent® 6530 Q-TOF LC/MS
column SeQuant ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 µm
mobile phase   [A] acetonitrile; [B] 50 mM ammonium acetate, pH as indicated with acetic acid in water (50 mM ammonium acetate, pH 6.93 in water not adjusted)
gradient 26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 9.9 min
flow rate 0.3 mL/min
pressure 986 psi
column temp. 40 °C
detector Q-TOF (ESI-negative)
detector other XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, CTP-482, GMP-362,  GDP-442,  GTP-522, UMP-323, UDP-403, UTP-483
injection 10 µL

Description

Analysis Note ZIC-cHILIC is a densely bonded zwitterionic stationary phase with phosphorylcholine functional groups covalently attached to silica. The stationary phase has an overall 1:1 charge balance between the permanent negative phosphate groups close to the silica support and the distal positively quaternary ammonium groups. It has though a slight positive surface charge because of the spatial arrangement. A true zwitterionic stationary phase, with permanent strong acidic and basic functionalities, provide hydrophilic partitioning combined with weak ionic interactions and makes the phase virtually pH independent as shown in this analysis.
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Application No. ASC140218I

Materials

     
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