LC/MS Analysis of Ribavirin and Related Substances on SeQuant(R)ZIC-HILIC

LC/MS Analysis of Ribavirin and Related Substances on SeQuant®ZIC-HILIC

Conditions

column SeQuant® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Ã… (1.50661.0001)
column temp. 25 °C
mobile phase [A] acetonitrile; [B] 0.385 g of ammonium acetate in 1000 ml water (5 mM); (85:15, A:B)
flow rate 1.5 mL/min
pressure 1334 psi 92 Bar
sample Ribavirin CRS 1000 ppm, Ribavirin impurity A 5 ppm and Ribavirin impurity D 0.5 ppm
injection 10 μL
detector 220 nm cell: 13 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS

Materials

     
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