LC/MS Analysis of Sorbitol and Mannitol on SeQuant ZIC-HILIC

LC/MS Analysis of Sorbitol and Mannitol on SeQuant ZIC-HILIC

Conditions

column SeQuant ZIC-cHILIC, PEEK, 250 x 4.6 mm, 3 μm, 100Ã… (1.50662.0001)
column temp. 45 °C (oven), 40 °C (detector cell)
mobile phase [A] acetonitrile; [B] 0.1% ortho-phosphoric acid in Milli-Q® water; (90:10, A:B)
flow rate 1.5 mL/min
pressure 1682 psi (116 bar)
sample 5.0 mg/mL (5000 ppm) each of sorbitol and mannitol in diluent. (Diluent: Acetonitrile: 0.1% ortho-phosphoric acid in Milli-Q® water 75:25 (v/v))
injection 20 μL
detector Refractive Index (RI) (cell: 10 μL)

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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