LC/MS Analysis of Trimethoprim on SeQuant(R) ZIC-HILIC

LC/MS Analysis of Trimethoprim on SeQuant® ZIC-HILIC


column SeQuant® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Ã… (1.50661.0001)
column temp. 25 °C
mobile phase [A] acetonitrile; [B] 1.261 g of ammonium formate in 1000 ml water (20 mM); (90:10, A:B)
flow rate 1.0 mL/min
pressure 1500 psi 105 Bar
sample 100 ppm Trimethoprim RS
injection 10 μL
detector 280 nm cell: 13 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS


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