LC/MS Analysis of Tryptophan, Tyrosine, and L-DOPA on SeQuant(R) ZIC-HILIC

LC/MS Analysis of Tryptophan, Tyrosine, and L-DOPA on SeQuant® ZIC-HILIC

Conditions

column SeQuant® ZIC-cHILIC, PEEK, 100 x 4.6 mm, 3 μm, 100 Ã… (1.50660.0001)
column temp. 23 °C
mobile phase [A] acetonitrile; [B] 25 mM ammonium acetate, pH 6.8; (80:20, A:B)
flow rate 1.0 mL/min
sample Tryptophan (9 mg) Tyrosine (104 4 mg) 3 4 dihydroxyphenylalanine (97 5 mg) diluted in 100 mL diluent + 1 mL ortophosporic acid
injection 1 μL
detector 254 nm

Description

Featured Industry Life Science and Biopharma
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS

Materials

     
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