LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18

LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18


column apHera C18, 15 cm x 2.1 mm I.D., 5 µm particles (56100AST)
column temp. 65 °C
mobile phase [A] 0.1% formic acid water; [B] 0.1% formic acid in 98:2 (v/v) acetonitrile:water
gradient 50% B for 2 min; to 100% B in 2 min; held for 2 min
flow rate 0.4 mL/min
sample 300 ng/mL in (4:1) 1% formic acid acetonitrile:water
injection 5 µL
detector MS, ESI(+), MRM


Analysis Note The apHera C18 HPLC column packed with polymeric particles demonstrated excellent peak shape and rapid elution for these bulky, immunosuppressant compounds under conditions not permitted by silica-based HPLC columns. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, immunosuppressants, Immunosuppressants
Featured Industry Clinical Testing
Pharmaceutical (small molecule)
Legal Information apHera is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for HPLC


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