LC/MS/MS Analysis of Marine Toxins on Ascentis® Express Biphenyl

LC/MS/MS Analysis of Marine Toxins on Ascentis® Express Biphenyl

Conditions

instrument Agilent 1290 Infinity Sciex 3200 Qtrap
column Ascentis® Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 µm (64065-U)
mobile phase   [A] 2 mM ammonium acetate with 0.1% formic acid in acetonitrile:water (5:95), [B] 2 mM ammonium acetate with 0.1% formic acid in acetonitrile:water (95:5)
gradient 30% to 80% B in 3 min, to 100% B in 1 min, held at 100% B for 2 min, to 30% B in 0.1 min, held at 30% B for 3.9 min
flow rate  400 µL/min
pressure 270 bar
column temp. 40 °C
detector MS/MS ESI(+) and ESI(-), MRM (see Figure for transitions)
injection 4 µL

Description

Featured Industry Food and Beverages
Legal Information Ascentis is a trademark of Sigma-Aldrich Co. LLC
OmniSolv is a trademark of Merck KGaA, Darmstadt, Germany
Suprapur is a trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Biotoxins in shellfish have recently come under increased scrutiny.  The European Union (EU) has issued regulation changes requiring all shellfish commodities to be tested for levels of these toxins. Shown here is a rapid separation of lipophilic marine toxins. Separation was achieved in less than six minutes on an Ascentis Express Biphenyl column.
Application No. ASC061217D

Materials

     
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