LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis(R) Express C18 after Extraction with BioSPME using C18 SPME LC Tips

LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis® Express C18 after Extraction with BioSPME using C18 SPME LC Tips

Conditions

sample/matrix human plasma
desorption process 30 min with 200 μL of methanol
extraction 60 min with 1 mL sample
SPME fiber SPME LC Tips C18 (57234-U)
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
column temp. 40 °C
mobile phase [A] 0.1% formic acid in water ; [B] methanol
gradient 5% B to 70% B in 3 min, then to 90% B in 0.1 min, hold 90% B for 0.9 min
flow rate 0.5 mL/min
pressure 4351 psi (300 bar)
sample 10 ng/mL in methanol
injection 10 μL
detector MS/MS, ESI(+), MRM (See Figure for transitions)

Description

Analysis Note Sensitive method for analysis of testosterone in human plasma.
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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