LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5


column Ascentis Express  F5, 15 cm x 2.1 mm I.D., 2.7 µm particles (53571-U)
column temp. 40 °C
mobile phase [A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)
flow rate 0.4 mL/min
pressure 5178 psi (357 bar)
injection 2 µL, each compound 300 ng/mL in 40:60 (water:methanol)
detector ESI(+), 100-1000 m/z


Analysis Note The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, vitamins, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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