LC/MS/MS of Phospholipids on Ascentis® C18

LC/MS/MS of Phospholipids on Ascentis® C18


column Ascentis C18, 15 cm x 2.1 mm I.D., 3 µm particles (581365-U)
column temp. 25 °C
mobile phase 10 mM ammonium formate in [A] water; [B] methylene chloride; [C] methanol; (2:3:5, A:B:C)
flow rate 0.2 mL/min
pressure 1270 psi (87 bar)
sample 300 mg/L in mobile phase
injection 2 µL
detector MS, ESI(+), MRM as indicated on the figure


Analysis Note An LC/MS/MS is shown here for the separation and detection of phospholipids on an Ascentis C18 reversed-phase column with high separation efficiency and excellent peak shape.
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS


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