UHPLC Analysis of A Monoclonal Antibody System Suitability Standard on BIOshell(TM) IgG 1000 Ã… C4

UHPLC Analysis of A Monoclonal Antibody System Suitability Standard on BIOshell™ IgG 1000 Ã… C4

Conditions

column BIOshell IgG 1000 Ã… C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)
column temp. 75 °C
mobile phase [A] 70:30 0.1% TFA (water): 0.1% TFA (acetonitrile); [B] 50:50 0.1% TFA (water): 0.1% TFA (acetonitrile)
gradient 0% B to 50% B in 50 min; 50% B to 100% B in 10 min
flow rate 0.2 mL/min
pressure 1325 psi (91.4 bar)
sample MMMonoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water) A03
4
A034Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water) A034MMMonoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)
A03
injection 1 μL
detector UV, 215 nm

Description

Analysis Note This application demonstrates the ability of the BIOshell IgG 1000 Ã… C4 column to resolve different monoclonal antibody (mAb) variants. The mAb variants within this sample differ by their pattern of glycosylation.
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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