UHPLC Analysis of Barbiturates on Titan™ C18

UHPLC Analysis of Barbiturates on Titan™ C18


column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 °C
mobile phase [A] 0.1% ammonium acetate (not adjusted); [B] acetonitrile; (70:30, A:B)
flow rate 0.4 mL/min
pressure 7540 psi (520 bar)
detector UV, 230 nm


Analysis Note Analysis of a barbiturate mix using an MS-compatible mobile phase and a Titan C18 UHPLC column is shown here. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Pharmaceutical Analysis, Clinical, drugs of abuse, antidepressants, Antidepressants, antipsychotics, antiepileptic/antiseizure, Anticonvulsant/antiepiletic/antiseizure, Drugs of Abuse, Antipsychotics
Featured Industry Clinical
Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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