UHPLC Analysis of Cannabinoids on Ascentis® Express C18

UHPLC Analysis of Cannabinoids on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
gradient 50% B to 100% B in 10 min
flow rate 0.4 mL/min
pressure 6300 psi (434 bar)
sample 50 ng/mL each in methanol
injection 1 µL
detector UV, 280 nm


Analysis Note Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, and inflammatory diseases. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing. Shown here is the separation on an Ascentis Express C18 column.
Categories Analytical Chromatography, Pharmaceuticals, Herbal medicines, Drugs of Abuse
Featured Industry Food and Beverages
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


Related Links