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UHPLC Analysis of Human IgA on BIOshell(TM) IgG 1000 Ã… C4

UHPLC Analysis of Human IgA on BIOshell™ IgG 1000 Ã… C4

Conditions

column BIOshell IgG 1000 Ã… C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)
column temp. 75 °C
mobile phase [A] 0.1% TFA (water); [B] 0.1% TFA (acetonitrile)
gradient 30% B to 60% B in 10 min; 60% B to 100% B in 10 min
flow rate 0.2 mL/min
pressure 1425 psi (98 bar)
sample Human IgA, 100 μg/mL, 0.1% TFA (water)
injection 1.0 μL
detector UV, 215 nm

Description

Analysis Note Human immunoglobulin A (IgA) can exist in two forms: a monomer (~150 kDa) in the serum and a dimer (~300 kDa) in mucosal secretory tissues. Serum IgA (monomeric) functions as an anti-inflammatory antibody by interacting with CD89 on immune effector cells. Serum IgA was chromatographically analyzed on a BIOshell IgG 1000 Å C4 column and compared to a fully porous, 300 Å C4 column. The BIOshell IgG column displayed superior resolution and recovery over the competitor column.
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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