UHPLC Analysis of Nucleosides on Purospher(R) STAR RP-18 endcapped

UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped


column Hibar Purospher® STAR RP-18 endcapped, 150 x 4.6 mm, 3 μm (1.50470.0001)
column temp. 25 °C
mobile phase [A] 30 mM ammonium phosphate, pH 5.3 with ammonium hydroxide in water; [B] Methanol; 97.5 [A] / 2.5 [B]
flow rate 1.0 mL/min
pressure 3060 psi 211 Bar
sample 5 μg/mL of 12 nucleosides in 98:2 30mM ammonium phosphate pH 5.3 with ammonium hydroxide in water: methanol
injection 5 μL
detector UV 254


Analysis Note Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics.
Featured Industry Life Science and Biopharma
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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