UHPLC Analysis of Proteomics Retention Time Standard on BIOshell(TM) IgG 1000 Ã… C4

UHPLC Analysis of Proteomics Retention Time Standard on BIOshell™ IgG 1000 Ã… C4


column BIOshell IgG 1000 Ã… C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)
column temp. 65 °C
mobile phase [A] water (0.1% TFA); [B] acetonitrile (0.1% TFA
gradient 20% B to 60% B in 20 min
flow rate 0.3 mL/min
pressure 1080 psi (74.5 bar)
sample Proteomics Retention Time Standard (p/n MSRT2), 100 μg/mL, water (0.1% TFA)
injection 1.0 μL
detector UV, 215 nm


Analysis Note The BIOshell IgG 1000 Ã… C4 column combines the advantages of superficially porous particles (SPPs) with large (1000 Ã…) pore diameters for high efficiency separations. This application demonstrates the resolving power of the BIOshell IgG column by comparing the chromatographic performance of this column against a column packed with 1.7 μm fully porous particles (FPPs) with a pore diameter of 300 Ã…. Resolution between the different proteins is improved on the BIOshell IgG column in addition to the ability of the BIOshell IgG column to resolve post-translational modifications seen in two of the peaks that is not resolved on the FPP-packed column.
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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