UHPLC Analysis of SigmaMAb Universal Antibody Standard on BIOshell(TM) IgG 1000 Ã… C4

UHPLC Analysis of SigmaMAb Universal Antibody Standard on BIOshell™ IgG 1000 Ã… C4

Conditions

column BIOshell IgG 1000 Ã… C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)
column temp. 75 °C
mobile phase [A] 70:30 0.1% TFA (water): 0.1% TFA (acetonitrile); [B] 50:50 0.1% TFA (water): 0.1% TFA (acetonitrile)
gradient 0% B to 50% B in 50 min; 50% B to 100% B in 10 min
flow rate 0.2 mL/min
pressure 1325 psi (91.4 bar)
sample SigmaMAb (p/n MSQC4), 1 g/L, 0.05% TFA (water)
injection 1.0 μL
detector UV, 215 nm

Description

Analysis Note The BIOshell IgG column is uniquely optimized for the resolution of analytes with high molecular weights like proteins and antibodies. The SigmaMAb Universal Antibody Standard is a recombinant human monoclonal antibody with a molecular mass of ~150 kDa expressed in Chinese Hampster Ovary (CHO) cells. This application demonstrates the ability of the BIOshell IgG column to resolve impurities and other species within the main analyte peak. Superior resolution was attained on the BIOshell IgG column, along with better recovery of the antibody, over a conventional, 1.7 μm UHPLC column packed with fully porous particles (FPPs).
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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