UHPLC Analysis of Temsirolimus and Sirolimus on Titan™ C18

UHPLC Analysis of Temsirolimus and Sirolimus on Titan™ C18


column Titan C18 10 cm x 2.1 mm, 1.9 µm particles (577124-U)
column temp. 55 °C
mobile phase [A] 0.1% formic acid in water, [B] methanol
gradient 75% B for 2 minutes, 75% B to 90% B from 2 to 12 minutes, 90% B from 12 to 14 minutes.
flow rate 0.3 mL/min
pressure 3300 psi (228 bar)
sample 100 µg/mL each in 20% methanol
injection 2.0 µL
detector UV, 276 nm


Analysis Note The anticancer agents temsirolimus and sirolimus are shown here resolved, efficienttly and rapidly, on a Titan C18 UHPLC column. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Pharmaceuticals, Chemotherapy drugs
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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