UHPLC Analysis of Temsirolimus on Titan™ C18

UHPLC Analysis of Temsirolimus on Titan™ C18


column Titan C18 10 cm x 2.1 mm I.D., 1.9 um particles (577124-U)
column temp. 55 °C
mobile phase [A] 0.1% formic acid in water, [B] methanol
gradient 75% B for 2 minutes, 75% B to 90% B from 2 to 12 minutes, 90% B from 12 to 14 minutes.
flow rate 0.3 mL/min
pressure 3300 psi (228 bar)
sample 100 ug/mL in 10% methanol
injection 2 µL
detector UV, 276 nm


Analysis Note Intravenous drug for the treatment of renal cell carcinoma
Categories Analytical Chromatography, Chemotherapy drugs
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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