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UHPLC Analysis of Turmeric Curcumin Herbal Supplement on Ascentis® Express RP-Amide

UHPLC Analysis of Turmeric Curcumin Herbal Supplement on Ascentis® Express RP-Amide

Conditions

sample preparation Filter with 0.22 µm PVDF
sample preparation Sonicate 20 min
extraction Weigh approximately 100 mg of herbal supplement ad add 100 mL ethanol
column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50774-U)
column temp. 35 °C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient 40% B held for 1 min; 40 to 100% B in (6 min); 100% B held for 5 min
flow rate 1.0 mL/min
pressure 1270 psi (88 bar)
sample turmeric curcumin hernal supplement extract in ethanol dilute 1:1 with water
injection 5 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, Anti-inflammatories, Antioxidants
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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