UHPLC Analysis of Verapamil and Metabolites on Titan™ C18

UHPLC Analysis of Verapamil and Metabolites on Titan™ C18


column Titan C18 5 cm x 2.1 mm, 1.9 µm particles (577122-U)
column temp. 35 °C
mobile phase [A] 10 mM potassium phosphate, monobasic pH 3.0; [B] acetonitrile; (70:30, A:B)
flow rate 0.6 mL/min
pressure 4290 psi (296 bar)
sample 10 µg/mL in 70:30, water:methanol
injection 1 µL
detector UV, 200 nm


Analysis Note Verapamil is prescribed to treat high blood pressure, severe chest pain (angina), and irregular heartbeat (arrhythmia). It is therefore of clinical and pharmaceutical interest. Shown here is the rapid analysis of verapamil on a Titan C18 UHPLC column. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Calcium channel blockers
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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