UHPLC/MS Analysis of Insulin Analogs on BIOshell(TM) A160 Peptide C18

UHPLC/MS Analysis of Insulin Analogs on BIOshell™ A160 Peptide C18

Conditions

column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.0 μm particles (67243-U)
column temp. 75 °C
mobile phase [A] 75:25 (0.1% DFA in water: 0.1% DFA in acetonitrile); [B] 50:50 (0.1% DFA in water: 0.1% DFA in acetonitrile)
gradient 23% B for 7 min; 23% B to 100% B in 1 min; hold at 100% B for 7 min
flow rate 0.2 mL/min
pressure 2500 psi (172 bar)
sample Analog Mixture, 20 μg/mL, 6:3:1 methanol:water:acetic acid containing 0.1% rat plasma
injection 0.5 μL
detector ESI(+), TIC 100-3000 m/z

Description

Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS

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