Determination of Water Content in Celecoxib Using Karl Fischer Titration

Product Group


General Information concerning the product group


Pharmaceutical products are often characterized by complex formulations. Difficulties observed during Karl Fischer determination are often caused by the limited solubility. In some cases side reactions have to be considered. In dependance of composition and properties of the formulations, various measures are necessary for an undisturbed Karl Fischer determination. In pharmaceutical guidelines (USP, Ph Eur, DAB) the Karl Fischer titration is described as common method for water determination. For some substances special procedures can be found. The determination of mass loss as method for water determination is not recommended.

Special Information concerning the sample and the methods

Water determination in Celecoxib can be carried out without problems according to standard methods.

Titration one component system


188002 Aquastar® - CombiTitrant 2 - One component reagent for volumetric Karl Fischer titration, 1 mL = approx. 2 mg water

50 ml 188008 Aquastar® - CombiSolvent - Methanol-free solvent for volumetric Karl Fischer titration with one component reagents

Titration Parameters:
Stirring time: 60 s
Default titration settings, e.g.:
I(pol) = 20 - 50 µA, U(EP) = 100 - 250 mV
Stop criterion: drift < 20 µL/min

Sample size:

The titration medium is first placed into the titration cell and titrated dry by means of the titrant. Then the sample is added from a weighing boat (exact sample weight determination by weighing of weighing boat before and after addition) and the titration is started. For complete dissolution of the sample a stirring time of 60 seconds is recommended.


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