Analytical Method: Analytical Quality Assurance, Standard for Hydrogen peroxide

Cat.No. 109122  Potassium permanganate solution 0.02 mol/l
Cat.No. 107209  Perhydrol 30 % for analysis
Cat.No. 100716  Sulfuric acid 25 % for analysis
Cat.No. 116754  Water for analysis
Preparation of a stock solution:
Place 10 ml of Perhydrol 30 % in a calibrated or conformity-checked 100-ml volumetric flask and make up to the mark with distilled water. Transfer 30 ml (full pipette) of this solution to a calibrated or conformity-checked
1000-ml volumetric flask and make up to the mark with distilled water. The stock solution prepared according to this procedure has a concentration of approximately 1000 mg/l hydrogen peroxide.
Precise assay of the standard solution (stock solution) for hydrogen peroxide:
Pipette 50.0 ml (full pipette) of the hydrogen peroxide stock solution of approx. 1000 mg/l into a 500-ml conical flask, dilute with 200 ml of distilled water, and add 30 ml of sulfuric acid 25 %. Titrate with a 0.02 mol/l potassium permanganate solution until the color changes to pink.
Calculation of the exact content of the hydrogen peroxide concentration:
Consumption of potassium permanganate (ml) x 34.02 = content of hydrogen peroxide, in mg/l
Further investigational concentrations may be prepared from the stock solution exactly determined according to the procedure described above by diluting accordingly.
When stored in a cool place (refrigerator), the stock solution and the further diluted investigational solutions remain stable for one day.


For use with test kits:


Measuring range
in mg/l


MQuantTM Peroxide Test

5 - 50 H2O2


MQuantTM Peroxide Test

1 - 100 H2O2


MQuantTM Peroxide Test

100 - 1000 H2O2


Reflectoquant® Peroxide Test

0.2 - 20.0 H2O2


Reflectoquant® Peroxide Test

100 - 1000 H2O2


Spectroquant® Hydrogen Peroxide Cell Test

2.0 - 20.0 H2O2 
0.25 - 5.00 H2O2


Spectroquant® Hydrogen Peroxide Test

0.15 - 6.00 H2O2







Related Links