Analytical Method: Calcium in plant material

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 109748  Spectroquant® Photometer NOVA 30 or

Cat.No. 109751  Spectroquant® Photometer NOVA 60 or

Cat.No. 173632  Spectroquant® Colorimeter Move 100

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 114815  Spectroquant® Calcium Test
Cat.No. 109057  Hydrochloric acid 1 mol/l
Cat.No. 116754  Water for analysis
Cat.No. 114724  Empty cells 16 mm with screw caps
Sample preparation:
In a closed empty cell mix 50 mg of the dried plant material with 5 ml hydrochloric acid 1 mol/l and heat at the thermoreactor at 120 °C (alternatively 100 °C) for 60 minutes.
Transfer the cooled solution into a 25-ml volumetric flask, fill up to the volume with water for analysis and mix well. Should the solution appear turbid, filter before analysis.
Determine with the above mentioned test kit.

Calcium content in %  =   analysis value in mg/l Ca x 2.5
weight of sample in mg





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