Analytical Method: Nitrite in dried meat

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 109748  Spectroquant® Photometer NOVA 30 or

Cat.No. 109751  Spectroquant® Photometer NOVA 60 or

Cat.No. 173632  Spectroquant® Colorimeter Move 100

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 114547  Spectroquant® Nitrite Cell Test
Cat.No. 114776  Spectroquant® Nitrite Test
Cat.No. 104874  Potassium hydrogenphthalate for analysis
Cat.No. 116754  Water for analysis
Potassium hydrogenphthalate solution 2 %:
Dissolve 2 g potassium hydrogenphthalate in 100 ml of water for analysis.
Sample preparation:
Cut down the sample with a knife or with scissors and ground it in a mixer or an electric coffee-mill to a fine, fibrous powder. In a closed Schliff-Erlenmeyer flask stir 5 g of the grounded product with 95 ml water for analysis and
5 ml potassium hydrogenphthalate solution 2 % for 2 hours. Then separate the suspension in the centrifuge at 4000 turns per minute for 15 minutes. Filter the supernatant solution through a folded filter.
Determine with the above mentioned test kits.
Nitrite content in mg/kg NO2 = analysis value in mg/l NO2 x 20
Nitrite content in mg/kg NaNO2 = analysis value in mg/l NO2 x 30


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