Application Notes Reflectometry – Glucose in Pickling Brine of Sauerkraut

Reflectometric determination after reaction with glucose oxidase and peroxidase

Glucose in Pickling Brine of Sauerkraut (Reflectometry)Reagents

Cat.No. 116720 Reflectoquant® Glucose Test

Cat.No. 117246 Reflectometer RQflex® 20

This application note pertains to RQflex®20 and all discontinued instruments
(RQflex® 10, RQflex® plus)

 

Sample preparation

In a 250-mL volumetric flask mix 1 mL of pickling brine with distilled water and make up to 250 mL.

Analysis

Press the reflectometer START key and simultaneously place the test strip into the prepared sample solution (15 - 30 °C) for 15 seconds, ensuring that both reaction zones are immersed. Remove the strip and allow excess liquid to run off via the long edge of the strip on to an absorbent paper towel. Wait 60 seconds and then measure the strip in the reflectometer. The value [mg/l] will be stored automatically. (Please refer to the RQflex® operating instructions and the package insert of the Reflectoquant® Glucose Test.)

Calculation

Glucose content [mg/l] = Measured value [mg/l] x 250

 

Results – comparison with enzymatic method

Sample Reflectoquant® [g/L] Enzymatic [g/l]
1 13.5 14.0
2 11.8 12.0

Materials

     
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