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Classical Silica HPTLC Plates

Our high performance thin-layer chromatography (HPTLC) plates are the perfect choice for quantitative separation using instrumental HPTLC. The optimized, smaller particles enable significant higher speed, more efficiency and better sensitivity than classical TLC plates. The parallel separation of many samples per plate and an extremely high matrix-tolerance enables a separation time of 20 seconds (or less) per sample with almost no need for sample preparation, which makes HPTLC outstanding in cost-efficiency.

A broad variation of silica plates for HPTLC with several plate dimensions and backings with without fluorescent indicators are available.

Higher speed and sensitivity

Our classical silica HPTLC plates enable fast, quantitative analyses of complex samples for manual or instrumental use. These plates offer higher speed and sensitivity than conventional TLC plates – making them the best choice for sophisticated separations.

Features and benefits

  • Faster analysis, only 3 – 20 min for optimum separations
  • 5 – 10 fold higher sensitivity than classical TLC
  • Highly reproducible, focused bands for quantitative analysis
  • Gold standard for instrumental automation

Smaller Particles for Advanced Separations

Our HPTLC plates use an optimized silica gel 60 sorbent with a particle size of only 5 - 6 µm, compared to the 10 - 12 µm used in classical TLC. This enables a higher packing density and smoother surface. What’s more, band diffusion is reduced, yielding especially compact sample zones or spots. The smaller particle size and thinner layer (200 µm or 100 µm) result in significantly higher sensitivity and faster analysis.

 

Features of HPTLC versus classical TLC HPTLC Classical TLC  
Mean particle size 5 - 6 µm 10 - 12 µm
Particle size distribution 4 - 8 µm 5 - 20 µm
Layer thickness 200 µm (100 µm) 250 µm
Plate height 12 µm 30 µm
Typical migration distance 3 - 6 cm 10 - 15 cm
Typical separation time 3 - 20 min 20 - 200 min
Number of samples per plate < 36 (72) < 10
Sample volume 0.1 - 0.5 µl 1 - 5 µl
Detection limits: absorption 100 - 500 pg 1 - 5 ng
Detection limits: fluorescence 5 - 10 pg 50 - 100 pg

 

Comparison of classical TLC
versus HPTLC plates
  A. TLC B. HPTLC
Sample 1. N-alpha-dansyl-L-aspargine Mobile phase Ethyl acetate/methanol/propionic acid (20/10/3)
  2. alpha-dansyl-L-arginine Detection UV 366
  3. Dansyl-L-cysteic acid Sample volume 4 µm 0.3 µm
  4. N-Danysl-L-serine Migration distance 10 cm 5 cm
  5. Dansyl-glycine Analysis time 42 min 13 min 45 sec
  6. N-N-Didansyl-L-tyrosine - - -

Comparison of the separation of dansyl amino acids under identical conditions. The comparison clearly demonstrates that the HPTLC plate delivers sharper zones with shorter migration distances and faster analysis times. In addition the HPTLC plate allows these separation of twice the number of samples simultaneously.

 

A. Classical TLC silica gel 60 plate
Classical TLC silica gel 60 plate
B. HPTLC silica gel 60 plate
HPTLC silica gel 60 plate

 

Application areas

HPTLC plates are ideal for highly demanding quantitative analyses, such as:

  • Identity testing in the analysis of herbal medicines
  • Highly sophisticated quantitative separations of pharmaceutical drugs using instrumental analysis
  • Quality control or purity testing of complex samples in pharmaceutical drugs
  • Trace analysis of contaminants in food

Complete Range

Our classical silica HPTLC plates are available with glass or aluminum backing in a variety of formats to suit your requirements. Fluorescent indicators are also available: green fluorescent F254, or blue fluorescent acid-stable F254s. Both indicators fluoresce in UV light at an excitation wavelength of 254 nm.

AMD HPTLC plates

With an extra-thin layer of only 100 μm, AMD HPTLC plates are designed for even more demanding, automated multiple development (AMD) applications. The technique combines repeated development of the plate in the same direction with reproducible gradient elution. AMD HPTLC plates provide extremely narrow bands, allowing the complete resolution of up to 40 components over a distance of only 60 mm. The special properties of AMD HPTLC plates are highly beneficial for complex investigations, like the qualitative and quantitative analysis of pesticides.

Premium Purity HPTLC Plates

Premium purity HPTLC plates are optimized for high-performance, completely contamination-free separations, such as demanding pharmacopoeia applications. The plates are wrapped in plastic-coated aluminum foil, which prevents deposition of plasticizers that could appear as unknown extra zones when using medium-polar solvent systems.

Unmodified HPTLC plates

Product No. Description Layer Sorbent/ Coating Material Layer Thickness/ µm Format/ cm Support/ Backing Pack size/ Content
1.05641.0001 HPTLC silica gel 60 HPTLC silica gel 60 200 20 x 10 glass 50 plates
1.05631.0001 HPTLC silica gel 60 HPTLC silica gel 60 200 10 x 10 glass 25 plates
1.05633.0001 HPTLC silica gel 60 HPTLC silica gel 60 200 10 x 10 glass 100 plates
1.15696.0001 HPTLC silica gel 60 F254s HPTLC silica gel 60 F254s *2 200 20 x 10 glass 25 plates
1.05642.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 20 x 10 glass 50 plates
1.05628.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 10 x 10 glass 25 plates
1.05629.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 10 x 10 glass 100 plates
1.05616.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 5 x 10 glass 25 plates
1.05547.0001 HPTLC silica gel 60 HPTLC silica gel 60 200 20 x 20 aluminum 25 sheets
1.05548.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 20 x 20 aluminum 25 sheets
1.05556.0001 HPTLC silica gel 60 F254 HPTLC silica gel 60 F254*1 200 5 x 7.5 aluminum 20 sheets
1.15552.0001 HPTLC silica gel 60 WR F254s HPTLC silica gel 60 WR F254s*2 200 20 x 10 glass 25 plates
1.11764.0001 HPTLC silica gel 60 F254 AMD, extra thin HPTLC silica gel 60 F254 *1 , AMD 100 20 x 10 glass 25 plates
1.12363.0001 HPTLC silica gel 60 WR F254s AMD, extra thin HPTLC silica gel 60 WR F254s*2 AMD 100 20 x 10 glass 25 plates
1.05648.0001 HPTLC silica gel 60 F254 premium purity HPTLC silica gel 60 F254 *1premium purity 200 20 x 10 glass 25 plates

*1 F254 Fluorescent indicator
*2 F254s Fluorescent indicator, acid stable
WR: water-resistant