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Modified Silica HPTLC Plates

Our modified silica TLC and HPTLC plates offer additional selectivities and significantly broaden thin layer chromatography applications. This makes them the optimal choice for challenging separations that cannot be adequately solved by unmodified silica. Since most substances separated on modified silica plates are colorless, the majority of our plates contain the blue fluorescent, acid-stable UV indicator F254s. Samples that absorb shortwave UV at 254 nm are detected due to fluorescence quenching.

Overcome Challenging HPTLC Separations

Reversed-phase (RP) modified silica HPTLC plates provide additional selectivities and significantly broaden thin-layer chromatography applications. Hence, they are well suited for demanding HPTLC separations, and as a pilot technique for HPLC. RP-2, RP-8, and RP-18 plates are based on silica gel 60, modified with aliphatic hydrocarbons. The chain length, combined with the degree of modification, defines the plate’s ability to tolerate the water of the solvent system, and strongly affects retention. Using the same solvent system, migration time increases in the order: RP-2, RP-8, RP-18.

RP-2 HPTLC sorbents exhibit higher polarity and a high affinity to aqueous solutions, tolerating up to 80% water. In contrast, the longer carbon chains, RP-8 and RP-18, can be run with up to 60% water in the solvent system. The specially developed RP-18W HPTLC plate offers a lower degree of surface modification, hence it can be used even with 100% water in the solvent system.

Features and benefits

  • Results are less dependent on atmospheric humidity
  • Allow use of aqueous solvent systems
  • Ready correlation with HPLC
  • No catalytic activity towards non-stable compounds (e.g. oxidative degradation)

CN and Diol-Modified Plates

Normal-Phase and Reversed-Phase Separations

CN and Diol-modified silica plates are moderately polar, and suitable for both normal-phase and reversed-phase systems. The CN-modified plate is based on a silica gel 60 modified with a cyano propyl group, while the Diol-modified plate utilizes a silica surface modified by a vicinal diol alkyl ether. The dual characteristic of the CN plate enables unique two-dimensional separations by using the normal-phase mechanism in the first direction, followed by the reversed-phase mechanism in the second direction.

NH2-Modified Plates

Excellent Separation of Charged Compounds

Amino-modified NH2 plates provide weakly basic ion-exchange characteristics with extraordinary selectivity for charged compounds. These unique features enable the separation of compounds such as nucleotides, purins, pyrimidines, phenols and sulfonic acids using simple eluent mixtures. For many applications, NH2-modified silica plates offer an alternative to PEI cellulose. In addition, they allow reagent-free detection of certain compounds (e.g. carbohydrates) by thermochemical fluorescence activation.

Features and benefits

  • Results are less dependent on atmospheric humidity
  • Separation of highly non-polar and polar substances using aqueous solvent systems
  • No catalytic activity towards non-stable compounds (e.g. oxidative degradation)

Reversed-Phase (RP) Modified HPTLC Silica Plates

Product No. Description Layer Sorbent/ Coating Material Layer Thickness/ µm Format/ cm Support/ Backing Pack Size / Content
1.13726.0001 HPTLC Silica gel 60 RP-2 F254s Silica gel 60 RP-2 F254s*2 200 10 x 10 glass 25 plates
1.13725.0001 HPTLC Silica gel 60 RP-8 F254s Silica gel 60 RP-8 F254s*2 200 10 x 10 glass 25 plates
1.05914.0001 HPTLC Silica gel 60 RP-18 Silica gel 60 RP-18 200 20 x 10 glass 25 plates
1.14296.0001 HPTLC Silica gel 60 RP-18 W Silica gel 60 RP-18 W 200 20 x 10 glass 25 plates
1.16225.0001 HPTLC Silica gel 60 RP-18 F254s Silica gel 60 RP-18 F254s*2 200 20 x 10 glass 25 plates
1.13724.0001 HPTLC Silica  gel 60 RP-18 F254s Silica  gel 60 RP-18 F254s*2 200 10 x 10 glass 25 plates
1.13124.0001 HPTLC Silica gel60 RP-18 W F254s Silica gel60 RP-18 W F254s*2 200 10 x 10 glass 25 plates

*1 F254 Fluorescent indicator
*2 F254s Fluorescent indicator, acid stable
W: wettable with water

CN-, Diol- and NH2- Modified HPTLC Silica Plates

Product No. Description Layer Sorbent/Coating Material Layer Thickness/ µm Format/ cm Support/ Backing Pack size/ Content
1.16464.0001 HPTLC silica gel 60 CN F254s HPTLC silica gel 60 CN F254s*1 200 10 x 10 glass 25 plates
1.12668.0001 HPTLC silica gel 60 Diol F254s HPTLC silica gel 60 Diol F254s*1 200 10 x 10 glass 25 plates
1.05636.0001 HPTLC silica gel 60 Diol F254s HPTLC silica gel 60 Diol F254s*1 200 20 x 10 glass 25 plates
1.12572.0001 HPTLC silica gel 60 NH2 HPTLC silica gel 60 NH2 200 20 x 10 glass 25 plates
1.13192.0001 HPTLC silica gel 60 NH2 F254s HPTLC silica gel 60 NH2 F254s*1 200 20 x 10 glass 25 plates
1.15647.0001 HPTLC silica gel 60 NH2 F254s HPTLC silica gel 60 NH2 F254s*1 200 10 x 10 glass 26 plates
*1 F254s, Fluorescent indicator, acid stable