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ProteoChrom® HPTLC Plates

ProteoChrom® HPTLC plates are optimized for highly efficient, incredibly sensitive separations, especially for the analysis of peptides and protein digests. The plates’ enhanced separation and staining procedures. For maximum convenience, each ProteoChrom® package includes an insert sheet with detailed instructions for solvent systems, running conditions and staining solution.

ProteoChrom® HPTLC Silica Gel 60 F254s Plates

Based on an extra-thin layer of our high-performance silica gel, ProteoChrom® HPTLC silica gel 60 F254s plates provide highly efficient separation characteristics for 1-D analysis of peptides and protein digests. Thanks to their special binder composition, the plates are also highly stable in water, thus ideal for use with aqueous solvent systems. Up to 20 peptides can be resolved, and as little as 1-2 ng per band can be visualized.

ProteoChrom® HPTLC Cellulose Sheets

Alternatively, ProteoChrom® HPTLC cellulose sheets are based on an extra-thin layer of optimized microcrystalline cellulose. Specially created protocols for development and staining enable straightforward 2-D analysis in only 4 hours.

Features and benefits

  • Highly reproducible: Optimized separation & staining procedures
  • Convenient: Easy-to-follow, detailed protocols included
  • High detection sensitivity: Extra-thin layers of 100 μm
  • Highly stable in water: Ideal for use with aqueous solvent systems

ProteoChrom® HPTLC plates

Product No. Description Layer Sorbent/ Coating Material Layer Thickness/   µm Format/ cm Support / Backing Pack size / Content
1.05650.0001 ProteoChrom® HPTLC silica gel 60 F254s ProteoChrom® HPTLC silica gel 60 F254s*1 100 20 x 10 glass 25 plates
1.05651.0001 ProteoChrom® HPTLC Cellulose ProteoChrom® HPTLC Cellulose 100 10 x 10 aluminum 25 sheets

*1 F254s: fluorescence indicator, acid stable

Application Examples

2-D separation of single protein digests on ProteoChrom® HPTLC cellulose sheets
Cytochrome C tryptic digests were 2-D separated on a ProteoChrom® HPTLC cellulose sheet followed by either: (A) Fluorescamine staining, or (B) Ninhydrin staining.

Cytochrome C tryptic digests were 2-D separated on ProteoChrom® HPTLC Cellulose sheet

2-D separation of single protein digests on ProteoChrom® HPTLC cellulose sheets

1-D separation of single protein digests on ProteoChrom® silica gel plates
Tryptic digests of various proteins were separated on a ProteoChrom® HPTLC silica gel 60 F254s plate followed by either: (A) fluorescamine staining, or (B) ninhydrin staining.

Tryptic digests of various proteins were separated on a ProteoChrom® HPTLC Silica gel 60 F254s plat

1-D separation of single protein digests on ProteoChrom® silica gel plates

Separation of a tryptic digest of b-casein
The densitogram depicts a tryptic digest of b-casein separated on a ProteoChrom® HPTLC silica gel 60 F254s plate followed by fluorescamine staining, and scanned with a CAMAG TLC scanner III in fluorescence mode at UV 366.

Separation of a tryptic digest of b-casein

Densitogram of a tryptic digest of b-Casein