TLC Analysis of Aflatoxin in walnut samples on HPTLC Silica gel 60 F254

TLC Analysis of Aflatoxin in walnut samples on HPTLC Silica gel 60 F254

Condition

plate  1.05628 HPTLC Silica gel 60 F 254 10 cm x 10 cm (1.05628.0001)
eluent [A] chloroform; [B] acetone; (9:1, A:B)
migration distance  5 cm
chamber  normal chamber without chamber saturation
detector  UV 366 nm and scan with TLC Scanner III (CAMAG)

Description

Application Sample Prep: 100 μl walnut extract was spiked with 400 μl Aflatoxin standard. Application by spraying 6 mm bands with CAMAG ATS4

Materials

     
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